Skip to main content

Use of Molecular Tools to Identify Microbial Communities in a Full Scale Biotrickling Filter Treating Odors from a Municipal WWTP

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial

The use of biotrickling filters (BF) for municipal waste air treatment is an important and emerging application of cell immobilization technology. BFs utilize microbial cells that are attached to a medium inside the reactor, which then oxidize the odorous constituents to odorless compounds. Since the process relies almost completely on biological means, it is environmentally friendly and has a very low operating cost.

Most often BF technology is treated as a “black box” and little is known about the complex combination of different physic-chemical and biological phenomena comprising the process. In order to improve our understanding of the technology, it is essential to know the microorganisms responsible for the oxidation of the odor constituents present in municipal foul air streams.

Novel molecular biology tools and fingerprinting techniques are available to characterize microbial populations in BFs. These techniques have allowed microbial ecologists and environmental engineers to determine microbial community structures in environmental samples without the limitations of traditional approaches such as conventional morphological analyses (staining and microscopy), and culture-based techniques. The most common approaches used in bioremediation research are those based on the polymerase chain reaction (PCR) amplification of 16S rRNA genes.

In this study, the microbial ecology of a full scale, synthetic media BF system treating odors from a municipal sludge processing building was determined using PCR and Terminal Restriction Fragment Length Polymorphism (T-RFLP) and cloning/sequencing of the 16S rRNA gene. The results show that T-RFLP and cloning/sequencing can be effective tools to further the understanding of the complex microbial ecology inside BFs. Ultimately, such understanding can result in smaller, more efficient biotrickling reactors that effectively remove both inorganic and organic odors.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics

Keywords: 16S rRNA; BFs; biological odor control; cloning; molecular tools

Document Type: Research Article

Publication date: 2010-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more