Electrical impedance spectroscopy (EIS) has been extensively used over the last 20 years to facilitate the understanding of the conditions under which microorganisms from the Prokaryote and Fungi kingdoms appear, survive and reproduce. A chip scale, 10 gold micro-electrode array device
was used to correlate the time response of the impedance of mixed strains of thiosulfate oxidizing biofilms with their preferential direction and speed of growth, based on a proposed equivalent RC circuit that describes the electrical behavior of the biological system. The array can
be used for evaluating the effectiveness of cell attachment and growth promoters, spreading versus thickening of biofilms to understand biofilm dimensional evolution and architecture, and effectiveness of synthetic surfaces for enhancing biofilm support and growth.
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