Skip to main content

Molecular Based Characterization of the Microbial Ecology and Activity of Anammox Bioreactors

Buy Article:

$9.50 plus tax (Refund Policy)

Or sign up for a free trial

Anaerobic ammonium oxidation (anammox) is the biological oxidation of ammonia using nitrite as an electron acceptor to produce dinitrogen gas. Anammox requires 60% less oxygen and no external organic carbon compared to conventional biological nitrogen removal (BNR). Despite these significant advantages, full-scale installations of anammox are uncommon primarily owing to the lack of well established process monitoring and control strategies that result in stable anammox reactor performance. The primary aim of this study was to determine the impact of long term operation with actual anaerobic digestion centrate from a full-scale operational wastewater treatment facility on the community diversity of an anammox bioreactor. A secondary aim was to develop and apply molecular biomarkers based on structural and functional genes to permit inference of anammox population activity. Over long-term operation, bacteria related to C. Brocadia sp. 40 were consistently the dominant anammox bacteria present in the anammox reactor. There was also a marked shift in biomass morphology from discrete cells to granular clusters. This morphological shift was paralleled by a shift also to more stable nitrogen removal performance in general and the succession and establishment of bacteria related to the Chlorobi-Bacteroidetes superfamily. There was good correspondence between anammox reactor nitrogen removal performance and anammox bacterial concentrations. Biomarkers for anammox activity based on structural (16S-23S rRNA intergenic spacer region, ISR) and functional (hydrazine oxygenasehzo) genes were developed and compared. Based on responses to exposure to oxygen and nitrite mediated inhibition and correlation with nitrogen removal performancehzo expression was a more sensitive indicator of anammox activity than ISR.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics

Keywords: Anaerobic ammonium oxidation (anammox); molecular biomarkershzo mRNA; population dynamics

Document Type: Research Article

Publication date: 2009-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more