Evaluation of Effluent Proteins Towards Characterization of Effluent Organic Nitrogen

$17.50 plus tax (Refund Policy)

Buy Article:


Proteins comprise a significant fraction of effluent organic carbon and organic nitrogen, but have not been extensively characterized, thus their fate in the environment is unknown. We used proteomics to characterize the proteins in wastewater effluents from three activated sludge plants. Analyses on raw and 0.45μm filtered samples included solids, COD, nitrogen species, and proteins. Concentrated samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Enzyme proteolytic activities were also assessed. Total nitrogen and total protein were significantly correlated. Protein profiles showed some proteins that were produced during biological treatment and some that persisted through the process. Microfiltration processes will not effectively remove all proteins as indicated by the similar profiles of 0.45μm filtered secondary effluent and crude effluent. The detection of proteolytic enzymes in the secondary effluent implies that effluent-derived enzymes can degrade aquatic organic matter and possibly modulate nutrient cycling in receiving waters.

Keywords: Proteins; effluent; organic nitrogen; proteomics

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864709793954330

Publication date: January 1, 2009

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • ingentaconnect is not responsible for the content or availability of external websites



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more