If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Recovery and Growth of Bacteria Following Heat Stress

$17.50 plus tax (Refund Policy)

Buy Article:

Abstract:

Heat stress has been used as a method of killing bacteria for many years, and is the basis for federal regulations promulgated to reduce pathogens in biosolids (40 CFR 503). However, recent studies have shown heat stressed organisms able to reactivate and regrow .The purpose of this study was to examine bacterial response to heat stress over time and to evaluate the ability of bacteria to recover and grow. Washed Cultures of E.coli were placed into bottles containing sterile buffer and buffer amended with 1% nutrient broth. The bottles were then subjected to heat at 55°C for 4, 6, and 24hours. Contents of bottles were assayed over 10days for growth on non-selective agar plates using the spread plate method. All plates were negative for growth immediately after heat stress, however in the samples heated for 4hrs and 6hrs, recovery of E. coli was seen in buffer with nutrient broth after 24hrs. Recovery was also seen in buffer alone after 72hrs. Samples heated for 24hrs did not show the ability to recover. Further work is being conducted using lower initial densities of E. coli to assess the difference between recovery and re-growth. This research also is evaluating similar responses in Salmonella, Enterococci, and Klebsiella spp.

Keywords: Heat Stress to Bacteria; Regrowth; VBNC

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864709793955023

Publication date: January 1, 2009

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • ingentaconnect is not responsible for the content or availability of external websites

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more