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Sample Preparation of Wastewater and Dissolved Solids for Use in an Estrogen Responsive Cellular Bioassay

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Bioassay analysis of Endocrine Disrupting Chemicals (EDCs) in wastewaters allows for the determination of the full hormonal response of the sample, but presents unique sample preparation challenges due to the sensitivity of bioassays to experimental conditions. We developed a novel sample preparation method for liquid and solid phases of wastewaters for use in bioassay testing. Wastewater samples were analyzed using a MVLN bioassay, which quantitatively measures estrogen activity through the activation of the endogenous estrogen receptor. To demonstrate adequacy of the method, qualitative GC/MS analysis of a subset of samples was performed. Preliminary results indicate that this methodology is an effective technique for preparation of wastewater samples for hormonal analysis in cellular bioassays. This method has potential crossover applicability to other media including drinking water and sludges, as well as use in the development and testing of EDC treatment methods, and improved assessment of ecological and public health impacts of water discharges.
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Keywords: Endocrine Disruptors; MVLN Cells; Wastewater

Document Type: Research Article

Publication date: 2009-01-01

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