If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Substrate-bound Tyrosinase Electrode for a Pesticide Biosensor

$17.50 plus tax (Refund Policy)

Buy Article:

Abstract:

Enzyme electrodes hold great promise for on-line monitoring of environmental pollutants such as pesticides, but there has been a barrier to convenient use since they require substrate to activate or maintain enzyme activity. This manuscript describes an approach for substrate integrating enzyme activation and corresponding current response at the substrate-bound enzyme electrode. Tyrosinase (TYR) was activated by the use of reduced pyrroloquinoline quinine (PQQ) which was covalently bonded with the functionalized self-assembled monolayer (SAM) modified gold nanoparticles, the mechanism being identified with cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The sensitivity was enhanced by the use of gold nanoparticles and the tyrosinase activity was maintained and converted into current signals.

Keywords: Substrate-bound tyrosinase electrode; gold nanoparticles; pesticides; pyrroquinoline quinine

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864709793954727

Publication date: January 1, 2009

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • ingentaconnect is not responsible for the content or availability of external websites

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more