Activated sludge from a conventional biological wastewater treatment plant was inoculated into a sequencing batch reactor (SBR) to investigate the techniques and required conditions for the selection and enrichment of denitrifying phosphorus accumulating organisms (DNPAO). The SBR was
operated under varying anaerobic, anoxic and aerobic conditions for 3 months. It was demonstrated that enhanced biological phosphorus removal was established rapidly by the application of the Anaerobic/Aerobic process and the selection of DNPAO was achieved by introducing an anoxic phase.
With an extension of the anoxic period and increasing nitrate dosage, the community shift between aerobic phosphorus accumulating organisms (PAO) and DNPAO was observed. An approximate 60% DNPAO fraction was obtained in this experiment.
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