Skip to main content

Characterization of Extracellular Polymeric Substances by Combined Enzyme Hydrolysis and Size Exclusion Chromatography-Infrared Microscopy

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial


This study suggests a new way of characterizing extracellular polymeric substances (EPS) using size exclusion chromatography (SEC) coupled with infrared microscopy (μFTIR) before and after enzyme hydrolysis. Previous research has established advantages to the enzymatic treatment of biosolids, through degradation of EPS. The chromatographic fingerprints of EPS were significantly altered using three different enzyme types. Application of either a commercial enzyme mix or an amylase/cellulase combination led to disappearance of molecular weight fractions above 0.5kDa. However protease application changed the chromatographic profile unexpectedly, forming very high molecular weight fractions (>4000 kDa). μFTIR results indicated that these large molecules result from mineral phase association with lower molecular weight organic molecules. EPS characterization confirmed that specific enzymes degraded their target compounds. Although the effect of enzyme treatment was observable using conventional sludge characterization methods, the SEC-μFTIR method provided more specific information on chemical mechanisms responsible for enzyme effects.

Keywords: amylase/cellulase; enzyme hydrolysis; infrared microscopy; protease; size exclusion chromatography

Document Type: Research Article


Publication date: January 1, 2009

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • ingentaconnect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more