Skip to main content

Biohydrogen Production FromFood Processing Wastewater by a Mixed Culture

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial


A series of batch tests in stirred-tank bioreactor were conducted to determine optimal initial cultivation pH and temperature to elevate hydrogen production from fructose wastewater. Hydrogen production efficiency was evaluated using specific hydrogen production potential (SHPP) and maximum specific hydrogen production rate (SHPRm). The experiments of initial pH were conducted at pH 4–8 at 55°C. The experimental results indicated that the initial pH markedly affected HP and SHPRm, volatile fatty acids and distribution as well as butyrate/acetate (BHu/HAc). The optimal initial pH for hydrogen production was 6 with peak values of SHPP 166.8 ml-H2/g-COD and SHPRm 26.7 ml-H2/g-VSS-h for fructose wastewater. Molasses processing wastewater was shown to have higher SHPP (187.0 ml-H2/g-COD) and SHPRm (42.7 ml-H2/gVSS-h) than fructose wastewater at pH 6. The DGGE profiles indicated that molasses processing wastewater is a better source as a substrate than fructose wastewater for the growth of hydrogen-producing bacteria due to high staining intensity of bands.

Keywords: Biohydrogen; butyrate/acetate ratio; food processing wastewater; mixed culture

Document Type: Research Article


Publication date: 2009-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free ContentFree content
  • Partial Free ContentPartial Free content
  • New ContentNew content
  • Open Access ContentOpen access content
  • Partial Open Access ContentPartial Open access content
  • Subscribed ContentSubscribed content
  • Partial Subscribed ContentPartial Subscribed content
  • Free Trial ContentFree trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more