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Biohydrogen Production FromFood Processing Wastewater by a Mixed Culture

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A series of batch tests in stirred-tank bioreactor were conducted to determine optimal initial cultivation pH and temperature to elevate hydrogen production from fructose wastewater. Hydrogen production efficiency was evaluated using specific hydrogen production potential (SHPP) and maximum specific hydrogen production rate (SHPRm). The experiments of initial pH were conducted at pH 4–8 at 55°C. The experimental results indicated that the initial pH markedly affected HP and SHPRm, volatile fatty acids and distribution as well as butyrate/acetate (BHu/HAc). The optimal initial pH for hydrogen production was 6 with peak values of SHPP 166.8 ml-H2/g-COD and SHPRm 26.7 ml-H2/g-VSS-h for fructose wastewater. Molasses processing wastewater was shown to have higher SHPP (187.0 ml-H2/g-COD) and SHPRm (42.7 ml-H2/gVSS-h) than fructose wastewater at pH 6. The DGGE profiles indicated that molasses processing wastewater is a better source as a substrate than fructose wastewater for the growth of hydrogen-producing bacteria due to high staining intensity of bands.
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Keywords: Biohydrogen; butyrate/acetate ratio; food processing wastewater; mixed culture

Document Type: Research Article

Publication date: 01 January 2009

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