Skip to main content

Identifying Estrogenic Effects of Wastewater on Receiving Stream Trout

Buy Article:

$9.50 plus tax (Refund Policy)

Or sign up for a free trial

Perhaps the microconstituents of greatest concern in the aquatic environment are those that mimic or interfere with natural hormones and have the potential to disrupt the endocrine system of aquatic organisms. The most potent of these endocrine disrupting compounds (EDCs) may be the natural and synthetic forms of estrogen, estradiol and ethinyl estradiol respectively, that have been shown to induce measurable effects in fish at environmentally relevant concentrations (1–4 ng/L). Reported effects include vitellogenin synthesis in males, intersex characteristics at the cellular level in gonad tissues, and population level effects such as skewed ratios of female to male fish. Municipal wastewater has been identified as a primary source of these EDCs to the environment. Snyderville Basin Water Reclamation District (SBWRD) in Park City, Utah, is concerned about the potential estrogenic effects of their effluent on brown trout (Salmo trutta) and Bonneville cutthroat (Oncorhynchus clarki) in the receiving stream, especially during late summer months each year when low flows upstream of the discharge point cause the lower reaches of the stream to become effluent dominated. Carollo Engineers (Carollo) assisted SBWRD with a project designed to investigate the estrogenic potential of the effluent. This project consisted of two major components: a sentinel study of trout held in the effluent in a pen to observe whether vitellogenesis occurred in male fish, and a field survey of females to males in the brown trout population downstream of the treatment facility to observe whether there was a skewed sex ratio towards a greater number of female fish. For the sentinel study 100 rainbow trout (Oncorhynchus mykiss) were individually tagged, measured for length and weight, and blood plasma samples (0.5 mL) collected before and after a three week exposure period. Fifty fish were placed in a pen in the final effluent, and the other 50 fish were held as a negative control at a hatchery. Blood plasma was treated with an anticoagulant and a protease inhibitor and then assayed for vitellogenin using an ELISA kit. At the end of the exposure period, fish were anesthetized and subsequently measured for total weight, length, gonad weight, and examined by necropsy to determine gender. Results for vitellogenin, gonadosomatic indices, and general health were compared for the two test groups of sentinel fish. For the field study, 70 brown trout were collected by electrofishing and gender was determined by partial stripping of gametes or necropsy. This paper will present the results of both the sentinel and field studies, and describe the methods used to investigate the impacts of effluent on trout.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics

Keywords: Endocrine disruptors; effluent; sentinel study; sex ratio investigation; trout; vitellogenin

Document Type: Research Article

Publication date: 2009-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more