Skip to main content

Ability of Specific Enriched Denitrifying Culture to Utilize Other Carbon Sources

Buy Article:

$9.50 plus tax (Refund Policy)

Or sign up for a free trial

The addition of external carbon sources for denitrification becomes necessary for wastewater treatment plants in order to meet more stringent requirement of effluent nitrogen (N) discharge to surface waters. Price volatility and safety issues associated with methanol, the commonly used external carbon source, has motivated the investigation on other alternative compounds capable of efficiently supporting denitrification. One practical concern with selecting alternative carbon sources is the adaptation/acclimation time required for establishing the populations specifically capable of utilizing a certain carbon source. In this study, we evaluated the ability of specific acclimated biomass to instantly utilize other carbon sources by comparing the immediate shortterm utilization rate of various carbon sources by three lab-acclimated denitrifying populations enriched with three different carbon sources, namely acetate, methanol and MicroCm™. Moreover, a review of the biochemical pathways for anoxic metabolism of these carbons is presented to highlight the key enzymes/pathways involved in the utilization of each specific carbon compound, providing some insights into the ability of a specific carbon enriched denitrifying population to utilize other carbon sources. The microbial population structures and dynamics associated with various carbon-specific denitrifying enriched cultures were also investigated using Automated Ribosomal Intergenic Spacer Analysis (ARISA) that differentiates the bacterial species based on the length of the intergenic region between the 16S and 23S ribosomal RNA. This new ecological approach reveals the differences in the microbial community structures of different denitrifying cultures and correspondingly the different denitrification behavior observed.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics

Keywords: ARISA and Community structure; Biological nutrient removal; Carbon sources; Denitrification; MicroCmâ„¢; Nitrogen removal

Document Type: Research Article

Publication date: 2009-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more