Effect of Alkyl Benzyl Dimethyl Ammonium Chloride and Temperature on Nitrification
The inhibitory effect of alkyl benzyl dimethyl ammonium chloride (AB), a quaternary ammonium compound commonly used as a disinfectant, was assessed at room temperature (22 to 24°C) and AB concentrations between 2 to 20 mg/L using an enriched, mixed nitrifying culture. Ammonia oxidation was inhibited with increasing AB concentration and ceased at 15 mg AB/L. For all tested AB concentrations, AB was not degraded in the nitrifying culture within 4 days of testing because of the very low concentration of heterotrophic bacteria in the enrichment culture. Assuming non-competitive inhibition, the AB inhibition coefficient was estimated as 1.5 ± 0.9 mg AB/L, indicating that AB is a potent nitrification inhibitor. Nitrification tests were conducted at a temperature range of 24 to 10°C with and without AB at an initial concentration of 5 mg/L using an aerobic, mixed heterotrophic/nitrifying culture. In the absence of AB, short-term exposure of the culture at 10°C resulted in the slow removal of ammonia and the slow conversion of the resulting nitrite to nitrate. However, short-term exposure of a duplicate culture at 10°C amended with 5 mg/L AB resulted in even slower ammonia removal, while nitrite accumulated and less than 30% of the initial ammonia was converted to nitrate. Maintaining the aerobic mixed culture at 10°C for over one month and in the absence of AB, resulted in even slower nitrification kinetics compared to those measured when the culture was first exposed to 10°C. AB was degraded in all tests conducted with the heterotrophic/nitrifying culture, but the AB degradation rate decreased significantly as the temperature decreased to 10°C. These results confirm the negative impact of low temperature on the rate and extent of nitrification and the deleterious effect of quaternary ammonium compounds on the nitrification process.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
Document Type: Research Article
Publication date: 2008-01-01
More about this publication?
- Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed. A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.
WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
- Subscribe to this Title
- Membership Information
- About WEF Proceedings
- WEFTEC Conference Information
- Learn about the many other WEF member benefits and join today
- Ingenta Connect is not responsible for the content or availability of external websites