Validation of UV reactors is an established practice that has until recently relied on biodosimetry to define the reduction equivalent dose (RED) delivered across a targeted operating range. An important drawback of biodosimetry is the inability of the method to yield a measurement
of the dose distribution in a reactor. A new UV reactor validation protocol has been developed as an alternate to biodosimetry. Lagrangian actinometry using dyed microspheres measures the dose-distribution in a reactor, which, when integrated with the dose-response kinetics of a targeted pathogen,
predicts the log-inactivation of the targeted pathogen. Large scale validations have been conducted simultaneously with T1 and MS2 coliphage biodosimetry in conformance with the USEPA Ultraviolet Disinfection Guidance Manual. These data are presented as a demonstration of the Lagrangian
actinometry process in direct comparison to conventional biodosimetry techniques. Evaluating UV reactors in the context of the UVDGM RED bias shows that direct knowledge of a reactor's dose-distribution is critically important.
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