Annular gap bioreactors or Couette-Taylor bioreactors (CTB) were used to apply varying shear forces to the biomass in this study. The shear forces in these bioreactors were controlled by adjusting the rotational speeds, allowing the shear to be independent of aeration rates. Both the
inner-cylinder rotating CTB (i-CTB) and outer-cylinder rotating CTB (o-CTB) showed decreasing mean floc diameter as the shear increased. Denaturing gradient gel electrophoresis (DGGE) revealed that microbial communities from the activated sludge from both i-CTB and o-CTB were changing over
time and indicated the potential contribution of protozoa to the development of aerobic granules.
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