Skip to main content

Investigating the Fate of Activated Sludge Extracellular Proteins in Sludge Digestion Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Buy Article:

$9.50 plus tax (Refund Policy)

Or sign up for a free trial

The purpose of this study was to examine the fate of activated sludge extracellular proteins in batch anaerobic and aerobic digestion using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). We were particularly interested in studying the fate of proteins that are bound with different sludge cations. Extracellular polymeric substances (EPS) extracted by three cation-specific extraction methods, the cation exchange resin (CER) procedure for extracting Ca2++Mg2+, sulfide extraction for removing Fe, and base treatment (pH 10.5) for dissolving Al, were subjected to SDS-PAGE and the resultant protein profiles were compared before and after sludge digestion. The initial SDS-PAGE on undigested activated sludge showed different protein profiles by the three methods, suggesting that Ca2++Mg2+, Fe, and Al are bound with different characteristic EPS and play unique physio-biochemical roles in floc formation. The analysis of SDS-PAGE post sludge digestion revealed that CER-extracted proteins remained intact in anaerobic digestion while they were degraded in aerobic digestion. While the fate of sulfide-and base-extracted proteins in aerobic digestion was not clearly resolved, their changes in anaerobic digestion were well observed. Sulfide-extracted protein bands were reduced by anaerobic digestion, indicating that Fe-bound EPS were degraded under anaerobic condition. While parts of base-extracted proteins disappeared after anaerobic digestion, others became more extractable along with extraction of new proteins, indicating that base-extractable EPS are dynamic in anaerobic digestion. All these results suggest that the metal content in influent sewage have a significant impact on activated sludge characteristics and sludge digestibility. The potential application of SDS-PAGE and the three extraction methods for choosing a proper sludge pretreatment and digestion method are also discussed in this study.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: ACTIVATED SLUDGE; CATIONS; EPS; EXTRACTION; PROTEINS; SDS-PAGE; SLUDGE DIGESTION

Document Type: Research Article

Publication date: 2007-10-13

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more