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The purpose of this study was to use molecular techniques to quantify and characterize ammonia-oxidizing bacteria (AOB) communities in several Intermittent Cycle Extended Aeration Systems (ICEAS®), and examine whether there are relationships between AOB community structure and system operational parameters and performance. Total AOB concentrations measured at four plants ranged from 1.6×109–8.9×109 gene copies/L, with the specific detection of 4.6×107–6.1×108 for Nitrosomonas europaea,5.5×106–2.3×108 for Nitrosomonas oligotropha, and 2.9×107–8.9×108 for Nitrosospira sp. By difference between total AOB and these three specifically targeted groups, other AOB types were dominant in all of the plants except one. According to clone library results from each plant, two of the systems were dominated by Nitrosomonas urea-like AOB and one was dominated by Nitrosomonas nitrosa-like AOB. The highest copies of Nitrosomonas oligotropha, comprising 10% of the total AOB community, were observed at the plant with the lowest ammonia loading and effluent concentration, while Nitrosomonas urea-like AOB were dominant at the plant with the highest ammonia loading and effluent concentration.

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864707787973491

Publication date: January 1, 2007

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  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

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