APPLICATION OF AN IMMUNOASSAY FOR QUANTIFYING NITRITE OXIDIZING ENZYME TO WASTEWATER REACTORS
Abstract:Nitrogen pollution of surface water bodies is of paramount concern. Nitrification must be carefully monitored to both optimize and control reactor performance. While ammonia oxidation has been found to be the rate limiting step in nitrification (Bollman, et al., 2002), the oxidation of nitrite was selected for analysis. Currently, nitrite oxidation activity in an environmental reactor can only be measured indirectly by observing chemical conversion or counting target microorganisms. Both of these methods have serious shortcomings. The goal of this project was to develop a novel immunoassay for quantifying nitrite oxidizing enzymes present in a wastewater treatment bioreactor. Immunological methods offer the possibility of rapid, sensitive, and extremely specific quantification of bacteria in the environment (Aamand, et al., 1996). The immunoassay developed herein is expected to lead to a better quantitative insight into the nitrite oxidation activity of a wastewater treatment bioreactor.
Document Type: Research Article
Publication date: 2007-01-01
More about this publication?
- Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed. A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.
WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
- Subscribe to this Title
- Membership Information
- About WEF Proceedings
- WEFTEC Conference Information
- Learn about the many other WEF member benefits and join today
- Ingenta Connect is not responsible for the content or availability of external websites