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Nitrogen pollution of surface water bodies is of paramount concern. Nitrification must be carefully monitored to both optimize and control reactor performance. While ammonia oxidation has been found to be the rate limiting step in nitrification (Bollman, et al., 2002), the oxidation of nitrite was selected for analysis. Currently, nitrite oxidation activity in an environmental reactor can only be measured indirectly by observing chemical conversion or counting target microorganisms. Both of these methods have serious shortcomings. The goal of this project was to develop a novel immunoassay for quantifying nitrite oxidizing enzymes present in a wastewater treatment bioreactor. Immunological methods offer the possibility of rapid, sensitive, and extremely specific quantification of bacteria in the environment (Aamand, et al., 1996). The immunoassay developed herein is expected to lead to a better quantitative insight into the nitrite oxidation activity of a wastewater treatment bioreactor.

Document Type: Research Article


Publication date: 2007-01-01

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