Skip to main content

Identification of Thermodynamically Inaccessible Target Sites for Fluorescence In Situ Hybridization (FISH)

The full text article is not available for purchase.

The publisher only permits individual articles to be downloaded by subscribers.

or click here to sign up for a free trial

Abstract:

Understanding accessibility of the target site in fluorescence in situ hybridization (FISH) is critical for predicting the efficiency of hybridization. In this study, we investigated whether the free energy related to the unfolding of the target molecule (δG0 3) was a good predictor of target site accessibility. Using a theoretical analysis we identified potential problematic sites based on a generated δG0 3 map and experimentally studied one of those sites in detail. It was shown that although δG0 3 was a useful parameter for predicting accessibility, there was also a need for defining its limitations.

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864707787969540

Publication date: January 1, 2007

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Ingenta Connect is not responsible for the content or availability of external websites
wef/wefproc/2007/00002007/00000013/art00027
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more