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A laboratory-scale reactor study was conducted to characterize the differences in nitrifier diversity in side-stream reactors and to evaluate the potential for biomass input to stabilize nitrification at low temperatures and allow shorter solids retention times. Recovery and enrichment of nitrifying microorganisms in side-stream reactors without sludge retention treating anaerobic digester supernatant in batch and continuously-fed configurations was evaluated. Phylogenetic analysis of 16S rDNA clones isolated from the continuously-fed side-stream reactor clustered within two Nitrosomonas lineages, N. europaea and N. oligotropha, whereas clones isolated from a batch-fed reactor clustered only with the N. europaea lineage. Complete oxidation of ammonia to nitrate was observed in all main-stream reactors, as expected, during the initial phase of the experiment conducted at room temperature. As temperature was reduced to 10°C performance of laboratory-scale reactors and kinetic parameter estimates tend to suggest a marginal benefit from input of batch-fed biomass. Large doses of biomass from the continuously-fed side-stream reactor were unable to induce nitrification in a main-stream reactor subjected to a wash-out stress.

Document Type: Research Article


Publication date: 2007-01-01

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  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

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