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To understand the stability and reliability of Anammox-denitrification (denammox) process, effect of short term temperature (10–35°C) and organic carbon (1–800 mg/L) variation was explored in three laboratory scale reactors. Activated sludge and anaerobic granules were as used as inocula. Solid biodegradable plastic and propionate were used as external carbon sources. The FISH and PCR analysis were applied to confirm the presence of anammox bacteria. Results showed that reactor R1 exhibited the only conventional denitrification whereas reactors R2 and R3 consisted of denammox process. Reactor temperature was found to strongly influence nitrogen removal in denammox process. For example, decrease in experiment temperature by 10°C reduced the nitrogen removal efficiency by 40%. On the other hand, this process demonstrated >95% removal efficiency at <50% of the organic carbon demand in conventional denitrification. No negative effect of propionate and biodegradable plastic on removal performance was observed until C/N ratio 20. Comparison of recovery rate in the denammox and conventional denitrification reactors suggested that the anammox like activity of denitrifiers was observed in the denammox reactor. In summary, these results showed that denammox process using biodegradable plastic has high potential for complete nitrogen removal including nitrate produced in the anammox process.

Document Type: Research Article


Publication date: January 1, 2007

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  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

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