COMPARISON AND STANDARDIZATION OF E.COLI PHOTOREPAIR UNDER DIFFERENT LAMPS
Abstract:The effectiveness of UV disinfection is often reported without accounting for possible photorepair of microorganisms, although many microorganisms are known to repair their DNA after UV disinfection. The conditions of photorepair studies are not standardized and different lamp spectra and intensities are often used. The hypothesis that these lamp differences are significant in affecting the time-based repair results was tested. E.COLI was chosen as model microorganisms and four commercial lamps commonly used for photorepair experiments with intensities ranging between 117-678 μW/cm2 were examined. The effect of lamp intensity on photorepair rate was tested, and with increasing intensity the photorepair was faster. Sensitivity analyses were performed to assess effect of lamp spectra on photorepair rate, and interestingly even very small differences in lamp spectra resulted in different rates of E.COLI photorepair. Therefore when performing photorepair experiments, intensity of the light, the specific spectra of lamp and the time of exposure have to be monitored and reported throughout the experiment.
Document Type: Research Article
Publication date: January 1, 2007
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