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The Variation of Nitrifying Bacterial Population Sizes in a Sequencing Batch Reactor (SBR) Treating Low/Mid/High Concentrated Wastewater

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The purpose of this study was to correlate the population size of ammonia-oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) with nitrification performance under various operational conditions (chemical oxygen demand (COD) concentration, dissolved oxygen (DO), and hydraulic retention time (HRT)) and influent allythiourea (ATU) shock. The AOB (genera Nitrosomonas and Nitrosospira) and NOB (genera Nitrobecter and Nitrspira) communities were analyzed using fluorescence in situ hybridization (FISH). AOB and NOB accounted for 6.2±0.9% and 2.5±0.3% in total biomass, respectively. The population sizes of AOB and NOB varied with different levels of COD, DO and HRT. Nitrosomonas and Nitrospira were dominant nitrifying bacteria under conditions favorable for nitrification, while Nitrosospira outcompeted Nitrosomonas under adverse conditions (low [NH4 +], low DO, short HRT, and ATU shock), and Nitrobecter outcompeted Nitrospira at high substrate concentrations (COD and [NH4 +]). Under ATU shock that inhibited the oxidation of 4 + to NO2 , the AOB population was substantially reduced with the stepwise increase of ATU dosage, and led to a corresponding decrease of NOB population. There was a discrepancy between nitrifying bacterial populations and their functions. Although AOB outnumbered NOB in all tests and became more dominant at low DO and short HRT, NH4 + oxidation, instead of NO2 oxidation, was the rate-limiting reaction for nitrification and susceptible to the adverse conditions. The study demonstrated the importance of elucidating the shifts of nitrifying bacterial population in order to optimize process design and operation at different influent characteristics, aeration intensity, retention time, and potential influent toxic shock.

Document Type: Research Article


Publication date: January 1, 2006

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  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

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