Skip to main content

Uncoupling Cr(VI) Reduction in Mixed-Culture Biofilm Systems: Electron-Flow Pathway Analysis

Buy Article:

$9.50 plus tax (Refund Policy)

Or sign up for a free trial

The electron transport pathway for microbial Cr(VI) reduction was investigated using specific inhibitors of the trans-membrane enzymes in a culture of Escherichia coli ATCC 33456 grown in a coculture with the phenol degrader, Pseudomonas putida DMP-1. The analysis involved blocking the electron flow pathway through the inner cell membrane at specific enzyme locations using known metabolic inhibitors such as azide (N3 -) and 21-oligomycin A. Cr(VI) reduction resulted from the utilization of NADH as an intermediate electron donor. Therefore, the electron pathway through NADH-dehydrogenase and cytochrome system was targeted. Results showed that 2,4-DNP restored Cr(VI) reduction activity in a culture previously inactivated by 21 oligomycin A. However, Cr(VI) reduction under uncoupler could not be sustained for a long period probably due to cell death as a result of lack of maintenance. This research contributes a new understanding of the biochemistry of Cr(VI) reduction in living cells and a more accurate representation of Cr(VI) reduction for future kinetic modeling.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: 2006-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more