Skip to main content

The Role of Glutathione Mediated Oxidative Stress Response Mechanisms in Nitrifying Bacteria

Buy Article:

$9.50 plus tax (Refund Policy)

Or sign up for a free trial

Nitrification in activated sludge is especially susceptible to oxidative chemical toxins. Several oxidative stress response mechanisms exist in bacteria, and one highly conserved biomolecule involved with antioxidant activities is glutathione. In many Gram-negative heterotrophic bacteria, glutathione mediates the glutathione-gated potassium efflux (GGKE) response, which activates secondary stress responses that protect important intracellular components. A search of the genome of the Gram-negative ammonia oxidizing autotroph Nitrosomonas europaea revealed that glutathione synthase was present, while several key enzymes involved with glutathione reduction and GGKE are missing; however, other mechanisms that facilitate potassium efflux for oxidative stress protection may exist. Experiments were performed using the ammonia oxidizing bacterium N. europaea and the nitrite oxidizing bacterium Nitrospira moscoviensis. Pseudomonas aeruginosa PAO1 was used as a positive control. Concentrations of total and oxidized glutathione were measured in N. europaea after exposure to the oxidative chemical sodium hypochlorite. This data showed that glutathione was present but not oxidized to equivalent levels in N. europaea and P. aeruginosa. Additional batch experiments were performed and soluble potassium levels were monitored to observe increases associated with oxidant induced potassium efflux mechanisms. Results using N. europaea and Ni. moscoviensis suggest that neither bacteria effluxed potassium in response to the oxidants N-ethylmaleimide or sodium hypochlorite, indicating that no oxidative stress-induced potassium efflux mechanism exists in these nitrifiers. Coupled with known genomic information, the results of this study provide greater insight into why nitrification is so susceptible to process upset in biological wastewater treatment.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: 01 January 2006

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more