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A NEW APPROACH FOR THE MEASUREMENT OF DECAY, ACTIVE FRACTION OF BIOMASS AND EXTRACELLULAR POLYMERIC SUBSTANCES IN HYDROGEN-DRIVEN DENITRIFICATION

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Abstract:

A new method for measuring the active fraction of biomass, decay coefficient and concentration of extracellular polymeric substances (EPS) was developed for a steady-state culture. The method was used to determine microbial characteristics, decay and yield coefficients in a hydrogen-dependent autotrophic (hydrogenotrophic) denitrification system. It was found that steady-state biomass, operated at 20 day solids retention time, was composed of 41% active biomass, 25.6% cell debris and 33.4% extracellular polymeric substance. Results of 0.041 d−1, and 0.27 mg active biomass per NO3-N were obtained for the decay coefficient and true yield, respectively.

Document Type: Research Article

DOI: https://doi.org/10.2175/193864705783867215

Publication date: 2005-01-01

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