AN IMMUNOASSAY FOR QUANTIFYING NITRIFICATION ACTIVITY IN A SUBMERGED ATTACHED GROWTH BIOFILTER
Abstract:The objective of this research is to develop a rapid molecular biology-based method for monitoring the nitrification activity of a biofilm reactor. A pilot-scale submerged attached growth biofilter (SAGB) was developed as a single-unit system capable of achieving concurrent removal of carbonaceous organics and nitrogen from municipal wastewater. A biofilm model, AQUASIM, was used to assist reactor operation and optimization. However, the model simulation and calibration required information regarding population dynamics and metabolic kinetics within the multi-population biofilm. This paper presents an enzyme-linked immunosorbent assay (ELISA) for quantifying nitrification activity. The ELISA was developed using a monoclonal antibody (Hyb 153-3) recognizing both nitrite oxidoreductase of Nitrobacter and nitrite-oxidation system of Nitrospira. A Direct ELISA calibration curve was developed for quantifying to a high degree of accuracy and precision the amount of nitrite oxidizing enzyme present in a control sample of cell extract. The use of immunoassays should facilitate a better measurement of the nitrification kinetics within the SAGB and provide the basis for improved model calibration for biofilm-based BNR processes.
Document Type: Research Article
Publication date: January 1, 2005
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