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An accurate and reliable method is developed for the detection 17 natural and synthetic estrogens, 5 of which are also in the conjugated form. This method uses a 500 mg Varian C-18 solid phase extraction (SPE) cartridge, followed by derivatization with bis(trimethylsilyl)trifluoroacetamide containing 1% trimethylchlorosilane as the derivatizing agent to produce trimethylsilyl-derivatives. The derivatization step took 15 minutes at room temperature to produce reproducible derivatives. The samples are analyzed with a GC/MS. The detection limits for all compounds ranges from 0.03 μg L−1 to 7.6 μg L−1 using a sample size of 200 ml. When sample volume is adjusted to 3 L the most sensitive compound produces a reproducible GC/MS response at 30 pg L−1. Recoveries range from 71 – 101% of all hormones through the entire method with relative standard deviation (RSD) ranging from 0 – 14% (n=3).

Document Type: Research Article


Publication date: 2005-01-01

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