Skip to main content

ANALYSIS OF LOW LEVELS OF FREE AND CONJUGATED SNYTHETIC AND NATURAL ESTROGEN HORMONES IN WATER AND WASTEWATER

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial

Abstract:

An accurate and reliable method is developed for the detection 17 natural and synthetic estrogens, 5 of which are also in the conjugated form. This method uses a 500 mg Varian C-18 solid phase extraction (SPE) cartridge, followed by derivatization with bis(trimethylsilyl)trifluoroacetamide containing 1% trimethylchlorosilane as the derivatizing agent to produce trimethylsilyl-derivatives. The derivatization step took 15 minutes at room temperature to produce reproducible derivatives. The samples are analyzed with a GC/MS. The detection limits for all compounds ranges from 0.03 μg L−1 to 7.6 μg L−1 using a sample size of 200 ml. When sample volume is adjusted to 3 L the most sensitive compound produces a reproducible GC/MS response at 30 pg L−1. Recoveries range from 71 – 101% of all hormones through the entire method with relative standard deviation (RSD) ranging from 0 – 14% (n=3).

Document Type: Research Article

DOI: https://doi.org/10.2175/193864705783857135

Publication date: 2005-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more