ENZYMATIC REMOVAL OF PHENOL FROM WATER AND SUBSEQUENT INACTIVATION OF THE ENZYME LACCASE

Authors: Dasgupta, S.; Taylor, K. E.; Biswas, N.; Bewtra, J. K.

Source: Proceedings of the Water Environment Federation, WEF/A&WMA Industrial Wastes 2004 , pp. 7-21(15)

Publisher: Water Environment Federation

Buy & download fulltext article:

OR

Price: $17.50 plus tax (Refund Policy)

Abstract:

Laccase catalyzes the oxidation of phenols. The reaction products become insoluble polymers and are readily removed from the solution. The potential use of laccase in an alternative enzyme-based treatment technology to remove parent phenol from buffered distilled water at room temperature in continuously stirred batch reactors was evaluated. After optimizing the pH at an optimum pH, the effects of product formation, dissolved oxygen (DO) availability, hydrogen peroxide addition, and aeration on phenol removal were investigated. The loss of enzyme activity was observed for different substrate concentrations. The polymeric products formed were found to reduce the enzyme activity and therefore the turnover capacity drastically after a certain time. The turnover capacity of laccase (defined as mM of phenol converted per catalytic unit of laccase inactivated) was determined for different phenol concentrations. A reactor design concept was developed incorporating the findings related to enzyme inactivation due to the effects of polymer formation.

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864704784105760

Publication date: January 1, 2004

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • ingentaconnect is not responsible for the content or availability of external websites

Tools

Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page