Skip to main content

BIOCHEMISTRY OF THE ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL SYSTEMS: INVESTIGATION OF THE ENZYME SYSTEMS

Buy Article:

$9.50 plus tax (Refund Policy)

Or sign up for a free trial

The biochemistry of enhanced biological phosphorus removal (EBPR) has been extensively studied by numerous researchers. However, these studies did not make use of some of the biochemistry and chemistry tools, such as enzyme assays and NMR techniques, to explore the metabolism of phosphorus removal under defined conditions. In this study EBPR sludges cultivated in two separate UCT systems operated at 5 and 20°C were used for examination of the anabolic and catabolic reactions taking place during the anaerobic and aerobic stages of phosphorus removal. At both temperatures, glycogen metabolism was shown to be essential for EBPR, and that the reducing equivalents for PHA synthesis were obtained through the EMP pathway. Enzyme assays performed on biomass samples indicated that the branched TCA pathway was operative during anaerobic metabolism at 20°C because an excess of NADH was produced during glycolysis. Low temperatures were shown to slow down glycogen metabolism significantly, thereby providing a competitive advantage to poly-P metabolism. At 5°C, the slower glycogen metabolism did not produce an excess of NADH, the branched TCA pathway was not operative, and the pyruvate generated by glycolysis was sent through the glyoxylate cycle under anaerobic conditions. Aerobic metabolism was also investigated, and the glyoxylate pathway was shown to be dominant because it is a good means of conserving carbon by minimizing the CO2 loss from the system at 20°C. On the other hand, at 5°C, the TCA cycle enzymes were more strongly induced under aerobic conditions. Inhibitor studies were found to be inconclusive because activated sludge has such a complex extracellular matrix, which reduces the efficiency of inhibitor compounds. Interpretation of such studies must be made with caution. To isolate poly-P metabolism from glycogen, techniques other than enzyme inhibition must be developed.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics

Document Type: Research Article

Publication date: 2003-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more