Skip to main content

GENETIC AND BIOCHEMICAL MARKERS FOR ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial

Abstract:

The suitability of genes encoding polyphosphate kinase (ppk) as genetic markers for enhanced biological phosphorus removal (EBPR) was investigated. The enzyme PPK is thought to be responsible for polyphosphate accumulation during EBPR. Gene fragment clone libraries were constructed from four full-scale activated sludges, three of which were performing EBPR. These libraries were screened for unique sequence types, and representative gene fragments were sequenced. Extensive phylogenetic analyses were carried out to confirm that ppk sequences similar to those found in laboratory scale sequencing batch reactors were also found in full-scale EBPR sludges, but not in activated sludge from a conventional completely aerobic process. The successful detection of nearly identical (99% DNA sequence identity) sequences in the full-scale EBPR sludges suggests that this sequence type could be used as a highly specific genetic marker for EBPR organisms.

Document Type: Research Article

DOI: https://doi.org/10.2175/193864703784641063

Publication date: 2003-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more