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Nitrate in the anaerobic condition was supposed to be reduced to ammonia (ammonification). But recent researches showed that denitrification was possible according to kinds of substrate and COD/NO3-N ratio in the anaerobic condition. Denitrification was dominant using
acetate as a substrate and denitrification/ammonification ratio was changed using glucose as a substrate. Two UBF (Upflow sludge bed filter) were used for the simultaneous denitrification and methanogenesis using acetate and glucose as substrates. The effect of alkalinity was studied in
the anaerobic condition at the constant COD/NO3-N ratio. This study showed that denitrification and ammonification in the anaerobic condition could be controlled via the influent concentration of alkalinity making no difference to substrates.
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