@article {Simpson:2002:1938-6478:786,
	author = "Simpson, Joyce M. and Domingo, Jorge W. Santo",
	title = "MOLECULAR EVALUATION OF CHANGES IN PLANKTONIC BACTERIAL POPULATIONS RESULTING FROM EQUINE FECAL CONTAMINATION IN A SUB-WATERSHED",
	journal = "Proceedings of the Water Environment Federation",
	volume = "2002",
	number = "11",
	year = "2002",
	abstract = "Considerable emphasis has been placed on developing watershed-based strategies with the potential to reduce non-point-source fecal contamination. 16S-ribosomal-deoxyribonucleic-acid (rDNA) methods were used to examine a source of fecal contamination. Objectives: (1) to explore the use of denaturing-gradient-gel-electrophoresis (DGGE) with a primer set targeting a broad range of microorganisms for community perspective and (2) use a specific set to target a small portion of population to determine if there may be a useful alternative for source tracking. Fecal enterococci increased from (Log<SUB>10</SUB>) 1.4 CFU&#47;ml upstream to 8.1 CFU&#47;ml downstream. Upstream DGGE patterns varied more than downstream (C<SUB>s</SUB>=42&#37;&#177;13). Downstream samples had greater pattern similarity (C<SUB>s</SUB>=70&#37;&#177;11). Change in pattern similarity suggests that fecal bacteria altered microbial community structure locally and maintained downstream influence. Sequenced DGGE bands were <I>Bacteroides</I>, <I>Prevotella</I>, and uncultured rumen bacteria. Identification of bacteria within watersheds after fecal contamination may allow better selection of microorganism(s) for identifying fecal contamination sources to improve water quality.",
	pages = "786-795",
	url = "http://www.ingentaconnect.com/content/wef/wefproc/2002/00002002/00000011/art00061",
	doi = "doi:10.2175/193864702784900606"
}