MOLECULAR EVALUATION OF CHANGES IN PLANKTONIC BACTERIAL POPULATIONS RESULTING FROM EQUINE FECAL CONTAMINATION IN A SUB-WATERSHED
Abstract:Considerable emphasis has been placed on developing watershed-based strategies with the potential to reduce non-point-source fecal contamination. 16S-ribosomal-deoxyribonucleic-acid (rDNA) methods were used to examine a source of fecal contamination. Objectives: (1) to explore the use of denaturing-gradient-gel-electrophoresis (DGGE) with a primer set targeting a broad range of microorganisms for community perspective and (2) use a specific set to target a small portion of population to determine if there may be a useful alternative for source tracking. Fecal enterococci increased from (Log10) 1.4 CFU/ml upstream to 8.1 CFU/ml downstream. Upstream DGGE patterns varied more than downstream (Cs=42%±13). Downstream samples had greater pattern similarity (Cs=70%±11). Change in pattern similarity suggests that fecal bacteria altered microbial community structure locally and maintained downstream influence. Sequenced DGGE bands were Bacteroides, Prevotella, and uncultured rumen bacteria. Identification of bacteria within watersheds after fecal contamination may allow better selection of microorganism(s) for identifying fecal contamination sources to improve water quality.
Document Type: Research Article
Publication date: 2002-01-01
More about this publication?
- Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed. A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.
WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
- Subscribe to this Title
- Membership Information
- About WEF Proceedings
- WEFTEC Conference Information
- Learn about the many other WEF member benefits and join today
- Ingenta Connect is not responsible for the content or availability of external websites