RAPID DETECTION METHOD FOR FAST GROWING MICROORGANISMS IN LAKE DECATUR, ILLINOIS
Abstract:Dominant and fast growing bacteria were isolated from lake Decatur, Illinois, and identified by 16s rRNA sequencing. The identified indicator organisms were stained with 4, 6-diamidino-2- phenylindole (DAPI) under various staining conditions such as dye concentration, bacterial concentration, fixation and staining times. The DAPI-DNA complex in these tests showed an emission shift to a longer wavelength, and the fluorescence intensity of the complex was greater than with the pure DAPI solution. Fluorescence intensity increased as both fixation and staining times were increased, although fixation time had a less significant effect. The principles developed here can potentially be used as a rapid indicator of water and wastewater quality. This paper focuses on the results of fluorescence staining optimization tests that have been performed.
Document Type: Research Article
Publication date: January 1, 2002
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