Skip to main content

A Comparison of the Colitag™ Method and the Standard Methods for the Detection of Fecal Coliforms and Escherichia coli in Urban Creeks

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial

Fecal contamination in urban wet weather runoff is a major water quality issue. The traditional indicator for fecal contamination has been the coliform count. Recently, methods have been introduced for Escherichia coli(E. coli), a more specific indicator of fecal contamination. One such method is Colitag™ which detects both MUG-positive and MUG-negative E. coli in a single 24 hour test. Unlike other E. coli detecting methods, Colitag™ is not subject to interference from Aeromonas, a ubiquitous aquatic bacterium. We designed a research project to evaluate the convenience and equivalence of the Colitag™ method in a small multi-purpose laboratory. Our reference was the Standard Methods fecal coliform test, the technique currently approved by the U.S. Environmental Protection Agency. From September 2000 to March 2001, 9 wet weather samples and 13 dry weather samples were taken in duplicate from urban creeks in Berkeley, California. The water samples were analyzed by the Colitag™ method in our lab and by the Standard Methods in a certified commercial laboratory. The Colitag™ method was found to be as sensitive as the Standard Methods for detecting fecal coliforms; statistical analysis of parallel test results showed a strong linear correlation of 0.87 between the two methods, a relationship that held up well at both high and low fecal coliform counts. In addition, the Colitag™ method gave rapid and specific enumeration of E. coli and takes only 24 hours compared to up to 96 hours for the Standard Methods. Saving three days means that monitoring agencies could respond faster to sudden increases in E. coli and could therefore take immediate corrective action to ensure public safety.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics

Document Type: Research Article

Publication date: 2002-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more