EFFECT OF MEDIUM PRESSURE AND LOW PRESSURE ULTRAVIOLET SYSTEMS ON THE INACTIVATION OF SELECTED BACTERIOPHAGES
Abstract:Four bacteriophages, MS2, phi X-174, T4 and PRD-1 were used to assess the effectiveness of both the low pressure, low intensity (LPLI) and the medium pressure, high intensity (MPHI) ultraviolet light Collimated Beam Units (CBUs) on virus inactivation. Results indicate that bacteriophage MS2 and PRD-1 were the most resistant to UV inactivation when irradiated by both types of UV systems, giving an average D90 value (1 log reduction) at 21 mW sec/sq cm and a k value (inactivation rate) of approximately 0.05 sq cm/mW sec. The most sensitive bacteriophage to UV irradiation was T4, resulting in a D90 at 1 mW sec/sq cm and k value of 0.9 sq cm/mW sec after UV irradiation. The differences in UV susceptibility between the bacteriophages can be due to differences in their nucleic acid characteristics and/or the structural makeup of the virus. The MPHI lamp used has an effective germicidal intensity that is approximately 4 times greater than that measured at 254 nm. Therefore, the exposure time needed to achieve the effective germicidal dose (dose necessary for a desired germicidal effect) is shorter for the MPHI as compared to the LPHI system. Additionally, the similar log survival curves generated by the irradiation of the 4 bacteriophages by the LPHI and MPHI UV lamps suggest that for the MPHI system, wavelengths in addition to that which is emitted at 254 nm is responsible for virus inactivation.
Document Type: Research Article
Publication date: 2002-01-01
More about this publication?
- Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed. A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.
WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
- Subscribe to this Title
- Membership Information
- About WEF Proceedings
- WEFTEC Conference Information
- Learn about the many other WEF member benefits and join today
- Ingenta Connect is not responsible for the content or availability of external websites