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Acclimation of biofilm cultures treating groundwater in Kumamoto, Japan, capable of anaerobic ammonium oxidation (Anammox) has been ongoing in our laboratories. Biofilms have exhibited a distinctly red color and simultaneous ammonium and nitrite removals under autotrophic, anoxic conditions, which are characteristics of anaerobic ammonium-oxidizing cultures. In this research, a maximum ammonium removal rate of 27 mg N/L/h was achieved, corresponding to a specific removal rate of 0.019 mg N/mg/h. Total-nitrogen removals were a factor of 1.9 over that of only ammonium, which is sufficiently high for industrial applications. For characterization of the bacterial composition of the biofilm, partial 16S rDNA sequences were amplified by PCR. Two of the phylotypes (consisting of 9 of 14 clones) were affiliated with Zoogloea and Aquaspirillum although their functions in the biofilm were not determined. Another sequence (5 of the 14 clones) had a close match to a planctomycete reported to have Anammox capability. These results demonstrate that an attached growth process can be effectively used to develop a continuous flow Anammox treatment process.

Document Type: Research Article


Publication date: January 1, 2001

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  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

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