Skip to main content


Buy Article:

$9.50 plus tax (Refund Policy)

Or sign up for a free trial

Analysis of fatty acid methyl esters (FAMEs) extracted from cellular membrane lipids presents exciting possibilities for monitoring and quantifying the abundance of NOCARDIA and possibly other organisms in activated sludge. We identified signature fatty acids unique to NOCARDIA by determining fatty acids associated with N. amarae. Two fatty acids (‘C19:1 alcoholω8’ and ‘SF3’) were identified as signature fatty acids for detecting and quantifying NOCARDIA in similar systems. In order to evaluate the ability of FAME technology to selectively quantify Nocardia levels in the mixed liquor samples, we extracted FAMEs from mixed liquor samples from various foaming and non-foaming full-scale treatment plants. Greater amount of the signature fatty acid (19:1 alcohol) was extracted from the mixed liquors containing higher levels of NOCARDIA. The peak areas of signature fatty acid in mixed liquor samples were closely correlated to the conventional filamentous counting technique. FAME profiles from chemostat culture of NOCARDIA showed that the distribution and quantity of the fatty acids were similar for two relatively short mean cell residence times; however the two of the seven fatty acids (16:0 and 19:1 Alcohol) were shifted at the higher cell residence time (4 days). This shift suggested that the FAME profile is dependent on the physiological state of the organism and it may be useful for monitoring the condition of organisms in activated sludge systems.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: 2001-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Learn about the many other WEF member benefits and join today
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more