Skip to main content

Development of DNA Microarray Technology for Environmental Applications

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial


Microarray technology is a powerful tool for parallel detection of genes and their expression in biological systems. Its potential in addressing environmental biotechnology issues is still unexplored. This study presents two key issues related to the use of microarray technology in mixed microbial systems: signal strength and specificity. A glass-based small DNA microarray was robotically printed using 17 full-length and 50 partial gene probes (80-120 bp long) of the pdt locus involved in encoding pyridine 2,6-bis-thiocarboxylate (PDTC), a molecule implicated in the transformation of carbon tetrachloride by Pseudomonas stutzeri strain KC. This array was tested for signal strength and specificity using Cy5 and Cy3 labeled DNA from pure cultures of strain KC and mixed communities. The results suggest that for the selected set of genes, partial and full-length probes may be equally specific under environmental conditions. However, the former may require alternative strategies to increase sensitivity because of lower signal strength.

Document Type: Research Article


Publication date: 2001-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more