MOLECULAR MICROBIAL ECOLOGY OF ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL IN AERATED-ANOXIC ORBAL™ PROCESSES
Abstract:The traditional process for enhanced biological phosphorus removal (EBPR) in wastewater treatment involves an anaerobic zone followed by an aerobic zone. Although there is no strict anaerobic zone in aerated-anoxic Orbal™ processes, phosphorus removal in excess of that required for cell growth does occur. The microbial ecology of polyphosphate accumulating organisms (PAO) in two full-scale Orbal™ wastewater treatment plants was investigated using flow cytometry to physically separate PAO from non-PAO and fluorescent in situ hybridization (FISH) to identify the main bacterial groups in the PAO subpopulation. Although organisms related to Rhodocyclus were present in these full-scale Orbal™ processes, they were not enriched in the PAO subpopulation, suggesting that the operational characteristics of aeratedanoxic processes select for a PAO community significantly different from that observed in traditional EBPR processes.
Document Type: Research Article
Publication date: January 1, 2001
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