Skip to main content

A COMPARISON STUDY OF TWO METHODS FOR PERFORMING NITRIFICATION INHIBITION TESTS

The full text article is not available for purchase.

The publisher only permits individual articles to be downloaded by subscribers.

or click here to sign up for a free trial

Abstract:

Control and monitoring of nitrification processes occurring in waste water treatment plants (WWTP) are crucial in preventing shock loads and inhibition of the sensitive nitrifying activated sludge population. The aim of this study was to compare two different methods for testing nitrification inhibition: an automated titrimetric Activity and NITrification Analyser (ANITA) and the traditional manual batch tests using 250 mL shakeflasks. Allyl thiourea (ATU), 3,5 dichlorophenol (3,5 DCP) and Dimethylformamide (DMF) were chosen as typical nitrification inhibitors. Activated sludge from a local WWTP was used for all the tests performed. In addition, classical photometric measurements of ammonia, nitrite and nitrate were performed for both methods. The Effective Concentration (EC) of toxicant which inhibits 50% (EC50) of the nitrification activity of the microbial population present in the system, was determined for the three test compounds chosen. The EC50 values obtained for ATU using both the ANITA (0.89 mg L−1), and batch shakeflasks (0.63 mg L−1) were in good agreement. For 3,5 DCP, EC50s obtained were 4.4 and 4.1 mg L−1 using the ANITA and the batch shakeflasks respectively, which are also in good agreement. The results obtained for DMF are not in agreement and the reason for the differences are discussed. In addition, results obtained using the ANITA compare well with results previously reported by other authors who used different methods (EC50s in the range of 0.5 to 2.0 mg L−1 for ATU and 0.5 to 3.6 mg L−1 for 3,5 DCP). In summary, the ANITA provides an automated, simple and sensitive method for testing nitrification inhibition, and it can be used to replace the manual testing methods provided the current equipment is improved.

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864701802779242

Publication date: January 1, 2001

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Ingenta Connect is not responsible for the content or availability of external websites
wef/wefproc/2001/00002001/00000014/art00015
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more