FEASIBILITY OF PAH BIODEGRADATION IN CONTAMINATED MARINE SEDIMENT UNDER AEROBIC AND SULFATE REDUCING CONDITIONS
PAHs have been shown to be very persistent in the environment, though their biodegradation in aerobic and anaerobic environments has been reported. Most published studies were conducted by spiking PAH free media with PAH compounds, and biodegradation was often tested by adding PAH - degrading pure cultures or enrichments. In this study the feasibility of PAH biodegradation under aerobic and sulfate reducing conditions by indigenous microorganisms present in the aged PAH-contaminated sediment was examined. The sediment was collected from the East River near Rikers Island, NY. The possibility of enhancing biodegradation by providing supplemental nutrients (N and P) to the aerobic studies, and by repeatedly adding ethanol or acetic acid as cosubstrate to the sulfate reducing studies was investigated. Sediment and overlaying seawater were used to prepare the sediment-water microcosms, with the sediment serving as the source of both the media containing PAH substrates and the microbial inoculum. Initial oxygen uptake for the aerobic biodegradation experiment was evaluated using respirometery. The results were used to establish a protocol for oxygen resupply to avoid oxygen depletion during the course of the experiment. With sufficient oxygen present, considerable degradation of the 2-, 3-, 4- and 5- ring PAHs was observed. Supplementing the N and P nutrients did not improve biodegradation, indicating that there was a sufficient supply of these nutrients already in the sediment. Under sulfate reducing conditions, phenanthrene was degraded while the sulfate concentration decreased appreciably. Phenanthrene removal was enhanced significantly by repeated addition of ethanol or acetic acid as cosubstrates. This may be due to the growth of phenanthrene – degrading organisms, and/or the stimulated cometabolism of phenanthrene. The phenanthrene-degrading sulfate reducing bacteria (SRB) did not appear to metabolize other PAHs, even those with a smaller number of benzene rings. The results of this study could be valuable for bioremediation of PAH-contaminated sediment.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
Document Type: Research Article
Publication date: 2001-01-01
More about this publication?
- Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed. A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.
WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
- Subscribe to this Title
- Membership Information
- About WEF Proceedings
- WEFTEC Conference Information
- Learn about the many other WEF member benefits and join today
- Ingenta Connect is not responsible for the content or availability of external websites