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Comparison of Methods for Detection of Salmonella in Biosolids: Initial Validation of USEPA Method 1682.

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There has been growing concern that the method currently used to monitor for salmonellae in biosolids may not adequately measure the presence of these organisms. USEPA is evaluating and validating an improved method for the recovery of salmonellae from biosolids (Method 1682), and has conducted a multiple-method comparison study. In this study, performance of an accepted method for Salmonella detection, using dulcitol selenite (DS) (EPA 625/R-92/013), was compared to the performance of three potentially improved methods: (1) a method using Selenite Brilliant Green (SBG) enrichment followed by passage onto XLD and MLIA agars, (2) a method using nonselective enrichment followed by culture into semisolid Rappaport-Vassiliadis (MSRV) medium followed by XLD, and (3) a method entailing initial nonselective enrichment with secondary selection in RV Broth followed by a 24-hour transfer to LMG. Typical colonies from the current method and the three alternative methods were subsequently confirmed by standard biochemical techniques. Results of this study, which was conducted at two laboratories over a 7-week period, indicate that all three alternate methods are more sensitive to the presence of salmonellae in biosolids than the currently approved method. Furthermore, the results indicate that the MSRV method is more sensitive to the presence of salmonellae in biosolids than the other three methods tested in the study.

Document Type: Research Article


Publication date: January 1, 2001

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