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THE RELATIONSHIP OF FILAMENT LEVELS TO FOAMING IN ACTIVATED SLUDGE DETERMINED BY OLIGONUCLEOTIDE PROBE HYBRIDIZATIONS

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The relationship between the levels of mycolic acid-containing actinomycetes (mycolata), Gordonia spp., and Gordonia amarae, and foam initiation and stability was characterized using (1) batch tests involving addition of G. amarae cells to activated sludge, and (2) analysis of a full-scale activated sludge plant that experienced seasonal foaming. Filament levels were quantified using group-, genus-, and species-specific oligonucleotide probes targeting the 16S rRNA of the mycolata, Gordonia, and G. amarae, respectively. Using batch tests, threshold Gordonia levels for foam formation and foam stability were determined to be 2 × 108 μm/ml, and 1 × 109 μm/ml, respectively. In the full-scale plant, the levels of Gordonia spp. and G. amarae increased during the course of foaming, and the foam formation threshold of 2 × 108 μm/ml corresponded to the onset of foaming.
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Document Type: Research Article

Publication date: 01 January 2000

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