IngentaConnect Study on electron transfer of fluorescent probe lumichrome and nu...

Authors: Li, Hui; Jiang, Zhi-Qin; Pan, Yang; Yu, Shu-Qin

Source: Research on Chemical Intermediates, Volume 32, Number 8, 2006 , pp. 695-708(14)

Abstract:

Laser flash photolysis (LFP) of lumichrome (LC) has been studied upon laser excitation at 355 nm. The transient absorption spectra of LC in aqueous solution displayed two bands at 380 nm and 540-700 nm for the triplet ³LC, and a wide band at 430-530 nm for the radical anion LC^˙. The triplet-state lifetime was measured up to 12 μs. It was found that the self-quenching of ³LC by the ground state of LC occurred to produce LC^˙ during LFP. The transient spectra of LC in the presence of electron-donating triphenylamine (TPA), nucleosides and nucleic acids showed that the absorbance of ³LC was strongly quenched by these substrates and the formation of LC^˙ was enhanced in a great extent simultaneously. The bimolecular quenching kinetics were studied and the rate constants of ³LC by a series of quenchers, including TPA, LC, nucleosides and nucleic acids were determined to be 10⁸-10⁹M⁻¹ s⁻¹. The photo-induced electron-transfer mechanism for these processes involving the triplet ³LC was proposed.

Keywords: LASER FLASH PHOTOLYSIS; ELECTRON TRANSFER; LUMICHROME; NUCLEOSIDES; NUCLEIC ACIDS

Document Type: Research article

DOI: 10.1163/156856706778606471

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Study on electron transfer of fluorescent probe lumichrome and nucleic acid by laser flash photolysis