Stem-cell culture on patterned bio-functional surfaces

Authors: Ruiz, A.¹; Buzanska, L.²; Ceriotti, L.¹; Bretagnol, F.¹; Coecke, S.¹; Colpo, P.¹; Rossi, F.¹

Source: Journal of Biomaterials Science, Polymer Edition, Volume 19, Number 12, 2008 , pp. 1649-1657(9)

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Abstract:

Bio-functional surfaces have been created by printing proteins on antifouling surfaces in a customised geometry. Human umbilical cord neural stem cells incubated on the samples readily attach to the protein defined domains, where they have been monitored during 21 days of culture. The stability of the pattern varies with the density of cells anchored to the microstamped proteins. Highly packed cell patterned domains favoured non-differentiated mode, while low-density areas allowed the spreading out of the cells and differentiation. Tailoring the geometry (pattern size and distances) enables improving the monitoring of the stem cells' developmental processes. The biocompatible surfaces can serve as a model to study processes accompanying stem cell neural lineage commitment.

Keywords: MICROPATTERNING; SOFT LITHOGRAPHY; CELL PATTERNING; HUCB-NSC

Document Type: Research article

DOI: http://dx.doi.org/10.1163/156856208786440514

Affiliations: 1: Institute for Health and Consumer Protection, Joint Research Centre, European Commission, TP 203, Via E Fermi, 21020 Ispra (VA), Italy 2: Institute for Health and Consumer Protection, Joint Research Centre, European Commission, TP 203, Via E Fermi, 21020 Ispra (VA), Italy; Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, Poland

Publication date: 2008-12-01