In vitro biocompatibility and degradation of terpolyester 3HB-co-4HB-co-3HHx, consisting of 3-hydroxybutyrate, 4-hydroxybutyrate and 3-hydroxyhexanoate

Authors: Liu, Qian; Chen, Guo-Qiang

Source: Journal of Biomaterials Science, Polymer Edition, Volume 19, Number 11, 2008 , pp. 1521-1533(13)

Buy & download fulltext article:

Price: $35.00 plus tax (Refund Policy)

Abstract:

A terpolyester consisting of 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB) and 3-hydroxyhexanoate (3HHx), abbreviated as P(3HB-co-4HB-co-3HHx), was studied for possible application as an implant biomaterial. L929 mouse fibroblasts, MC3T3-E1 murine osteoblasts and a human cell line of immortalized human keratinocyte (HaCat cells) were used to study the biocompatibility of P(3HB-co-4HB-co-3HHx). Cell morphology and cell activity were studied using scanning electron microscopy (SEM) and the MTT assay, respectively. All three cell types showed higher activities when grown on films of P(3HB-co-4HB-co-3HHx) compared with their growth on poly(lactic acid) (PLA), co-polyester PHBHHx films and on polylysine-coated plates (blank), respectively. The three cell types grown on the terpolyester also demonstrated a well-spread cell shape and large number of pseudopods due to strong cell-cell and cell-material interactions. It was clearly observed that P(3HB-co-4HB-co-3HHx) had a much faster degradation rate than PHBHHx after 15 weeks of incubation in phosphate-buffered saline under dynamic conditions. The results proved that the terpolyester had favorable biocompatibility and biodegradability compared with the well-studied polyesters PLA and PHBHHx.

Keywords: PHB; POLYHYDROXYALKANOATES; PHBHHX; TERPOLYMER; BIOCOMPATIBILITY; TISSUE ENGINEERING

Document Type: Research article

DOI: http://dx.doi.org/10.1163/156856208786140391

Affiliations: 1: Multidisciplinary Research Center, Shantou University, Shantou 515063, Guangdong, China

Publication date: 2008-11-01