In vitro and in vivo analysis of co-electrospun scaffolds made of medical grade poly(ε-caprolactone) and porcine collagen

Authors: Chen, Z.C.C.¹; Ekaputra, A.K.²; Gauthaman, K.¹; Adaikan, P.G.¹; Yu, H.³; Hutmacher, D.W.⁴

Source: Journal of Biomaterials Science, Polymer Edition, Volume 19, Number 5, 2008 , pp. 693-707(15)

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Abstract:

In this study, a nanofiber mesh made by co-electrospinning medical grade poly(ε-caprolactone) and collagen (mPCL/Col) was fabricated and studied. Its mechanical properties and characteristics were analyzed and compared to mPCL meshes. mPCL/Col meshes showed a reduction in strength but an increase in ductility when compared to PCL meshes. In vitro assays revealed that mPCL/Col supported the attachment and proliferation of smooth muscle cells on both sides of the mesh. In vivo studies in the corpus cavernosa of rabbits revealed that the mPCL/Col scaffold used in conjunction with autologous smooth muscle cells resulted in better integration with host tissue when compared to cell free scaffolds. On a cellular level preseeded scaffolds showed a minimized foreign body reaction.

Keywords: PCL; COLLAGEN; SMOOTH MUSCLE CELLS; CORPUS CAVERNOSA; NANOFIBER; ELECTROSPINNING; SCAFFOLDS

Document Type: Research article

DOI: http://dx.doi.org/10.1163/156856208784089580

Affiliations: 1: Department of Obstetrics and Gynecology, National University of Singapore, Singapore 2: Graduate Program in Bioengineering, Faculty of Medicine, National University of Singapore, Singapore 3: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Institute of Bioengineering and Nanotechnology, A*STAR, Singapore 4: Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, Australia

Publication date: 2008-05-01