Synthesis, characterization and stability of a luteinizing hormone-releasing hormone (LHRH)-functionalized poly(amidoamine) dendrimer conjugate
Authors: Bi, Xiangdong1; Shi, Xiangyang2; Baker, James R.2
Source: Journal of Biomaterials Science, Polymer Edition, Volume 19, Number 1, 2008 , pp. 131-142(12)
Publisher: VSP, an imprint of Brill
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Abstract:
Cancer targeting is crucial for cancer detection, therapy and targeted drug delivery. A dendrimer-peptide conjugate has been synthesized based on poly(amidoamine) dendrimer generation 5 (PAMAM G5) as a platform and a luteinizing hormone-releasing hormone (LHRH) peptide as a targeting moiety. The synthesized conjugate was fully characterized using nuclear magnetic resonance (NMR), UV-Vis spectrometry, reverse-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Further stability experiments showed that the synthesized conjugate was stable after 72-h incubation in phosphate-buffered saline (PBS) buffer (pH 7.4) at 37°C. The synthesized conjugate may find applications in biomedical targeting, gene delivery and imaging.Keywords: PAMAM DENDRIMER CONJUGATE; LHRH PEPTIDE; STABILITY
Document Type: Short communication
DOI: 10.1163/156856208783227686
Affiliations: 1: Department of Physical Sciences, Charleston Southern University, Charleston, SC 29406, USA 2: Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI 48109, USA
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